• 生物活性
  Natamycin (pimaricin)是抗真菌化合物。它是一种天然存在的三唑类抗真菌药物。具有极少或无味道干扰。

靶点及简介 ：

Antifungal macrolide polyene

  Natamycin 特异性结合麦角固醇存在于模型膜上。Natamycin 在生长被阻止的条件下不改变酵母细胞膜的渗透性。[1] Natamycin 阻止分离的液泡的融合而不损害液泡膜的屏障功能。Natamycin 干扰了蜂窝液泡形态，造成在酵母细胞中的许多更小的液泡结构的形成。Natamycin 抑制了液泡融合的起动阶段。[2] Natamycin显示对暗色真菌，Curvularia种低的MIC。[3] Natamycin和γ-环糊精（NT-γ何秀兰的）形成复合物以增加NT在水溶液中的溶解度和稳定性，并降低了药物的副作用，而不会降低抗真菌活性。[4] Natamycin和Natamycin-gamma CyD 复合物的MIC90低于0.0313 毫克/毫升，这表明gamma CyD有效地提高Natamycin的抗真菌活性，从而表明了Natamycin-gamma CyD 复合物的临床应用价值。[5]  

Natamycin is a naturally-occurring macrolide polyene antifungal agent produced during fermentation by the bacterium S. natalensis, commonly found in soil. With minimal inhibitory concentrations ranging from 4-64 μM, natamycin is used to treat fungal infections, including Candida, Aspergillus, Cephalosporium, Fusarium, and Penicillium.[1],[2],[3] Natamycin blocks fungal growth by binding specifically to ergosterol with an apparent affinity of ~100 μM, but it does not permeabilize cell membranes as other polyene antibiotics are known to do.[2] Natamycin is also used in the food industry as a preservative.[4]

Reference:

[1]. Lalitha, P., Vijaykumar, R., Prajna, N.V., et al. In vitro natamycin susceptibility of ocular isolates of Fusarium and Aspergillus species: Comparison of commercially formulated natamycin eye drops to pharmaceutical-grade powder. Journal of Clinical Microbiology 46(10), 3477-3478 (2008).

[2]. te Welscher, Y.M., ten Napel, H.H., Balagué, M.M., et al. Natamycin blocks fungal growth by binding specifically to ergosterol without permeabilizing the membrane. The Journal of Biological Chemisty 283(10), 6393-6401 (2008).

[3]. Dong, X.H., Gao, W.J., and He, X.P. Antifungal efficacy of natamycin in experimental fusarium solani keratitis. Int.J.Ophthalmol. 5(2), 143-146 (2012).

[4]. de Vries, R.P., de Lange, E.S., Wösten, H.A.B., et al. Control and possible applications of a novel carrot-spoilage basidiomycete, Fibulorhizoctonia psychrophila. Antonie Van Leeuwenhoek 93, 407-413 (2008).

参考文献：

[1]. Wu H, et al. SlnM gene overexpression with different promoters on natamycin production in Streptomyces lydicus A02. J Ind Microbiol Biotechnol. 2014 Jan;41(1):163-72.

[2]. Eady RA, et al. A pilot trial of natamycin. A topical antifungal agent. Practitioner. 1969 Nov;203(217):670-3.

• 溶解度及制备方案
  
  

DMSO	100 mg/mL   (150.21 mM)
Water	Insoluble
Ethanol	Insoluble


制备储备液

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.47 mL	7.33 mL	14.67 mL
5 mM	0.29 mL	1.47 mL	2.93 mL
10 mM	0.15 mL	0.73 mL	1.47 mL
50 mM	0.03 mL	0.15 mL	0.29 mL